Human Retrovirus Protocols: Virology and Molecular Biology (Methods in Molecular Biology) A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods
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| Title | : | Human Retrovirus Protocols: Virology and Molecular Biology (Methods in Molecular Biology) |
| Author | : | |
| Rating | : | 4.90 (751 Votes) |
| Asin | : | 1588294951 |
| Format Type | : | Hardcover |
| Number of Pages | : | 494 Pages |
| Publish Date | : | 2005-05-17 |
| Genre | : |
Editorial : "More than 20 years after the discoveries of HTLV and HIV, there appears to be a need for a consolidated reference of many of the standard and not-so-standard protocols used to characterize the human retroviruses. This book, containing some 37 detailed protocols provided by multinational authors in two parts, does an admirable job. Part I, therefore, includes guidance on isolation of HTLV, HIV and Human Foamy Virus (HFV) from peripheral blood, lymph nodes, CNS tissue and genital secretions (semen, vaginal fluids). It also describes a variety of molecular methods (largely PCR-based) to assess viral levels, integration status and drug sensitivity, plus quantification by antigen detection. At some 313 pages, Part I forms the substantial portion of the book. Part II (168 pages) guides on assessing cell and receptor tropism, construction and utilization of molecular clones and also use of microarrays to look at host and viral gene expression. The book on the whole is well illustrated with c
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Bortz. The text and line drawings are clear. And yet, though she had plenty of down days and shed plenty of tears, Loretta somehow maintained her indomitable spirit and her determination to not only survive the trauma of the illness and the amputations of her limbs, but to thrive and use her own experiences to help others.
I'm giving this book a 5-star rating because Loretta's life story is so inspirational and learning about her successes--and sorrows--will help anyone who is going through unexpected illness, loss, amputations, or any other tragedy. I wanted to hear more about the issues of Co-Dependency and that is covered briefly on the first side, but then it is more about loving yourself, relaxing, breathing, stretching, etc. De Beauvoir retorted, "the problem is not to make men happy but find the reason for their existence" to which the student replied, "it is easy to see you have never been hungry".
What kept me reading was the concise and often poetic writing st
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